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Stabilization of correctly situated isthmotectal connections is hypothesized to occur because ACh, which the isthmotectal axon releases, causes a boost in glutamate release by the coactive retinotectal axons. The extra glutamate promotes opening of more NMDA receptors on the retino-recipient dendrite, which in turn releases enough neurotrophin to stabilize the nearby isthmotectal axon.
