Figure 3.
Activation of mGluR2,3 suppresses sIPSC activity in dLGN relay neurons. A, Representative current recording from a dLGN relay neuron in the presence of TTX (1 μm) illustrating the suppressive action of mGluR2,3. The mGluR2,3 agonist APDC (10 μm) reduces the IPSC frequency (b) compared with baseline activity (a) in a reversible manner (c). The APDC-mediated reduction in sIPSC activity is blocked in the presence of a specific mGluR2,3 antagonist, LY341495 (200 nm, d). Representative current traces in each condition are shown in an expanded time scale in the left panel. The expanded traces on the right correspond to the underlined region in the left traces. B, The graph illustrates the time course of the APDC-mediated alterations in sIPSC frequency in the absence and presence of LY341495. Each bar represents the number of events in consecutive 30 s bins. C, Cumulative probability (Cum. Prob.) graphs reveal a significant increase in interevent intervals (a) by APDC without altering the sIPSC amplitude (b). D, Population data illustrating the concentration-dependent decrease in sIPSC frequency by APDC (a), but there is no change in sIPSC amplitude (b). Numbers within the bar graph indicate the number of cells tested. The data are plotted as mean ± SD (*p < 0.05).