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. 2006 Dec 27;26(52):13505–13514. doi: 10.1523/JNEUROSCI.3970-06.2006

Figure 2.

Figure 2.

Immunostaining of rat brain with phosphospecific antibodies. Rat brain sections were double immunofluorescence stained with S563P or S603P (red) together with K89/34 (Total; green). Images were taken by a fluorescence microscope equipped with ApoTome. A, Cerebral cortex. Note that some large pyramidal neurons in the layer V were stained more intensely than other neurons in the region. Scale bar, 50 μm. B, Pyramidal neurons in the subiculum. Overlaid images show the heterogeneity of S563 and S603 phosphorylation in Kv2.1 clusters. Scale bar, 10 μm.