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Recombinant human CNTF. Recombinant protein was purified on nickel columns. After electrophoresis on 10% acrylamide gel and visualized by SimplyBlue SafeStain (Invitrogen), CNTF protein was detected as a single band of ∼23 kDa. Lane 1, Protein molecular weight marker (molecular weight was indicated on the left for the 2 bands close to CNTF protein). Lane 2, Purified recombinant human CNTF protein, 1 μg. Lane 3, Purified recombinant human CNTF protein, 5 μg.
