Figure 5.

Mutagenesis of the Egr4^KCC2 element decreases Egr4-mediated induction of the KCC2(−309/+42) reporter construct in N2a cells. a, The C nucleotide at position 4 in Egr4^KCC2 that deviates from the Egr consensus sequence is shown in gray. Two G nucleotides modified by site-directed mutagenesis are indicated with an asterisk. b, Mutagenesis of the two G critical nucleotides in Egr4^KCC2 significantly prevents Egr4-induced activation of the KCC2(−309/+42)^Egr4mut reporter. c, Insertion of two Egr4^KCC2 sites in the endogenous (reverse), but not in the opposite (forward), orientation just upstream of the minimal KCC2(−180/+42) promoter enables the reporter to be activated by Egr4 (*p < 0.05; ***p < 0.001; Student's t test; n = 4). Error bars indicate SEM.