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. 2006 Oct 18;26(42):10796–10807. doi: 10.1523/JNEUROSCI.2746-06.2006

Figure 4.

Figure 4.

Kainate receptor antagonist reduces facilitation at synapses onto EGFP-expressing SOM interneurons. A, Examples of EPSCs recorded from an s. radiatum EGFP-expressing SOM interneuron before (green) and during (magenta) application of the GluR6-specific kainate receptor antagonist NS-102 (20 μm). Calibration: 25 ms, 50 pA. B, Group results for paired-pulse ratios versus interval from EGFP-expressing SOM interneurons before (solid green circles) and during application of NS-102 (open magenta circles) (n = 6). NS-102 caused a significant decrease in paired-pulse facilitation for intervals from 20 to 60 ms (paired t test, p < 0.05). C, Group results for five-pulse ratios versus frequency from EGFP-expressing SOM interneurons before (solid green circles) and during application of NS-102 (open magenta circles) (n = 10). NS-102 causes a significant reduction in the five-pulse ratio at frequencies of 20 and 50 Hz (paired t test, p < 0.05). D, Examples of EPSCs recorded from an s. radiatum non-EGFP interneuron before (black) and during (gray) application of the GluR6-specific kainate receptor antagonist NS-102 (20 μm). Calibration: 25 ms, 120 pA. E, Group results for paired-pulse ratios versus interval from non-EGFP interneurons before (solid black triangles) and during application of NS-102 (open gray triangles) (n = 5). NS-102 had no effect on the paired-pulse ratio in non-EGFP interneurons (paired t test, p > 0.05). F, Group results for five-pulse ratios versus frequency from non-EGFP interneurons before (solid black triangles) and during application of NS-102 (open gray triangles) (n = 5). NS-102 had no effect on the five-pulse ratio in non-EGFP interneurons (paired t test, p > 0.05). G–I, NMDA EPSCs were isolated by blocking AMPA receptors with GYKI 52466 (50 μm). The non-selective AMPA/kainate antagonist DNQX (10 μm) was used to block kainate receptors. Filled symbols, Recorded for control; open symbols, recorded with DNQX applied. G, Group results (n = 5) obtained from EGFP-expressing SOM interneurons show that blocking kainate receptors with DNQX reduced the paired-pulse ratio of the NMDA EPSC for 25, 50, and 100 ms interpulse intervals (paired t test, p < 0.05). H, I, Group results show that there was no effect of DNQX on the paired-pulse ratio for either non-EGFP interneurons (H; n = 6) or pyramidal cells (I; n = 5) (paired t test, p > 0.05).