Figure 5.

The effect of BMP2 misexpression on the expression of topographic molecules along the D-V axis. Coronal section in situ hybridization of the retinas of E8 embryos after DOX treatment is shown. RCASDC/rtTA and RCAN-TRE/BMP2 were simultaneously electroporated at E1.5. Control retinas and transfected retinas are shown on the left and right, respectively. Boxed regions were selected at the ventral limit with the expression of the topographic molecules in the control retina (A–E) and the ventral retina (F–I) to show the alteration by the manipulation. The expression of Tbx2 (A), Tbx3 (B), Tbx5 (C), ephrin-B1 (D), and ephrin-B2 (E) was upregulated and expanded in the retina, whereas that of Ventroptin (F), cVax (G), EphB2 (H), and EphB3 (I) was downregulated.