Table 1.
Primer pairs used for detection of TRPC channel transcripts
Channel subtype | Primer sequence | Expected size | Intron in amplicon | Gene location |
---|---|---|---|---|
TRPC1–5′ | GGGTCCATTACAGATTTCAA | 207 bp | 1 | chr 3 |
TRPC1–3′ | AAGCAGGTGCCAATGAACGA | |||
TRPC3–5′ | GTATGTGGACAGTTACGTC | 553 bp | 2 | chr 4 |
TRPC3–3′ | CTACATCACTGTCATCCTC | |||
TRPC4–5′ | TGGGATGGCGGACTTCAG | 391 bp | 1 | chr 13 |
TRPC4–3′ | ATGCCTTTGCAGGTTAACCC | |||
TRPC5–5′ | GTGGAGAAGGGGGACTATGC | 525 bp | 1 | chr X |
TRPC5–3′ | CCTCACTTGATAAGGCAATG | |||
TRPC6–5′ | CTCTGAAGGTCTTTATGC | 428 bp | 1 | chr 11 |
TRPC6–3′ | TCATCCTCAATTTCCTGG | |||
TRPC7–5′ | AACCCAGCGTTTACAACG | 361 bp | 1 | chr 5 |
TRPC7–3′ | ATGAGGCACATCTTGATTC |
Primers were designed according to sequences available from the European Molecular Biology Laboratory database. At least one intron region was included in the amplicons to avoid amplification of genomic DNA and unprocessed RNA. Both 5′ primers and 3′ primers are written in 5′ to 3′ direction. chr, Chromosome.