Skip to main content
. 2006 Oct 18;26(42):10667–10676. doi: 10.1523/JNEUROSCI.3253-06.2006

Figure 7.

Figure 7.

Modulation of GAD protein expression in the ventrum area of SI cortex over the nursing period. A, Typical immunoblots for the GAD 67 and 65 proteins in nursing (N) and non-nursing (nN) rats at days 6 and 21 postpartum. Actin controls show that loading was equal for each lane. B, C, Mean ± SD values of ID obtained from quantitative analysis of Western blots in each group of animals. The integrated optical density (ID) values obtained for GAD immunoblots were normalized to the ID of corresponding immunoblots for actin. Significant differences between nursing (filled squares) and non-nursing (open squares) and between 6 and 21 d postpartum are indicated. No change was recorded in non-nursing mothers (B). Both GAD 67 and 65 proteins were upregulated after 6 and 12 nursing days but not after 21 d. In lactating rats, levels of GAD 67 and 65 proteins gradually decreased to control levels from 6 d postpartum, as daily nursing time decreased (C). Statistically significant differences are indicated (Newman–Keuls post hoc comparisons).