Figure 2.
Binding of Myo1c fragments to stereociliary receptors. Each panel shows a single confocal section containing ∼10 hair bundles of a whole-mount bullfrog sacculus. Each group of three panels depicts the following: left, fluorescently conjugated phalloidin labeling of filamentous actin; middle, bound recombinant Myo1c fragments; right, overlay of actin (red) and Myo1c binding (green). A, Binding of Myo1c-T701 to receptors at the tips of stereocilia. B, Excess CaM abolishes Myo1c-T701 binding to stereociliary receptors. C, Myo1c-T701IQ2Mut binding to stereociliary receptors. D, Excess CaM does not block interactions of Myo1c-T701IQ2Mut with stereociliary receptors. E, Myo1c-N2 does not bind to stereociliary receptors. Scale bar: (in E) 5 μm.