Figure 5.

Myo1c binding to stereociliary receptors in the mouse organ of Corti requires intact tip links. Each panel shows a single confocal section containing inner and outer hair cells from a whole-mount mouse organ of Corti. Left column, Fluorescently conjugated phalloidin labeling of filamentous actin; middle column, bound recombinant Myo1c fragments; right column, overlay of actin (red) and Myo1c binding (green). A, Binding of Myo1c-T701 to mouse cochlear hair cells. B–D, Treatment of the organ of Corti with 5 mm BAPTA, EGTA, or La³⁺ results in the loss or marked reduction of Myo1c-T701 binding to stereociliary tips. Scale bar: (in D) 10 μm.