Table 2.
Membrane characteristics of bladder afferent neurons in vehicle-treated and NGF-treated rats
| Vehicle | NGF |
||
|---|---|---|---|
| 1 week | 2 week | ||
| Neurons with TTX-resistant spikes | |||
| No. of cells | 30 | 34 | 42 |
| Diameter (μm) | 24.1 ± 1.6 | 26.8 ± 1.4 | 29.9 ± 1.5* |
| Input capacitance (pF) | 30.4 ± 1.6 | 34.1 ± 2.5 | 42.8 ± 3.4** |
| RMP (mV) | −54.5 ± 2.3 | −54.0 ± 2.1 | −53.5 ± 1.8 |
| Spike threshold (mV) | −21.1 ± 1.1 | −25.4 ± 1.6* | −28.1 ± 1.7** |
| Spike duration (ms) | 8.1 ± 0.5 | 7.51 ± 0.6 | 6.9 ± 0.4 |
| No. of spikes (600 ms depolarization) | 1.3 ± 0.2 | 7.7 ± 2.2* | 11.6 ± 1.8** |
| Capsaicin sensitivity | 28/30 (93%) | 32/34 (94%) | 39/42 (93%) |
| Neurons with TTX-sensitive spikes | |||
| No. of cells | 12 | 13 | 16 |
| Diameter (μm) | 34.1 ± 1.7** | 35.2 ± 1.5 | 35.8 ± 1.3 |
| Input capacitance (pF) | 47.2 ± 2.2** | 46.2 ± 2.8 | 47.9 ± 2.6 |
| RMP (mV) | −57.2 ± 2.9 | −58.0 ± 2.1 | −56.9 ± 2.6 |
| Spike threshold (mV) | −29.3 ± 1.5** | −29.8 ± 1.7 | −30.1 ± 1.6 |
| Spike duration (ms) | 5.2 ± 0.7* | 5.0 ± 0.5 | 5.1 ± 0.6 |
| No. of spikes (600 ms depolarization) | 11.3 ± 2.1** | 12.2 ± 1.6 | 12.3 ± 1.8 |
| Capsaicin sensitivity | 1/12 (8%) | 1/13 (8%) | 2/16 (12%) |
RMP, Resting membrane potential.
*p < 0.05,
**p < 0.01 compared with the value in bladder afferent neurons with TTX-resistant spikes from vehicle-treated rats.