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. 2006 Nov 8;26(45):11807.

Figure 6.

Figure 6.

PSD-95 stops cypin-promoted dendritic branching. A, Hippocampal neurons were transfected with cDNAs encoding the indicated constructs on DIV 10. Scale bar, 10 μm. B, Dendrite number was assessed at 12 DIV. Overexpression of PSD-95-DsRed1 decreased cypin-promoted increases in primary and secondary dendrite number (cypin + PSD-95). PSD-95 decreased secondary dendrite number below basal (GFP) levels regardless of cypin overexpression. n values are as follows: GFP, 33; GFP + PSD-95-dsRed, 10; cypin, 30; cypin + PSD-95-dsRed, 20. *p < 0.05 and ***p < 0.001 by Kruskal–Wallis test followed by Dunn’s multiple-comparisons test. C, Left, Overexpression of PSD-95 decreases the proportion of primary dendrites that branch (ANODEV, p = 0.0003). PSD-95 blocks the effect of cypin (p = 0.81). Right, Cypin increases the number of excess secondary dendrites (ANODEV, p = 0.00002), and overexpression of PSD-95 blocks this effect. Error bars indicate SE.