Skip to main content
. 2006 Feb 22;26(8):2174–2183. doi: 10.1523/JNEUROSCI.3799-05.2006

Figure 5.

Figure 5.

SALM1 colocalizes with the NMDA receptor. Neurons at 14 DIV were transfected with SALM1-myc (A) or SALM1-Δ4myc (B) and were permeabilized and stained with anti-NR2A antibody to detect endogenous NMDA receptors. The area of colocalization of SALM1 and NR2A (C) and the average punctum size of NR2A (D) were analyzed. There is a dramatic reduction in colocalization of SALM1-Δ4myc and NR2A (13.4 ± 2.9%) compared with SALM1-myc and NR2A (83.3 ± 28.3%) (C). The area of colocalization between SALM1 and NR2A is defined as the percentage of SALM1-myc or SALM1-Δ4myc that has NR2A staining. The average punctum size of NR2A in SALM1-myc-transfected neurons (30.3 ± 5.5 pixels) is larger than that in SALM1-Δ4myc-transfected neurons (12.2 ± 4.5 pixels) (D). *p < 0.001. Scale bar, 10 μm. Data were obtained 48 h after transfection. For SALM1-myc and SALM1-Δ4myc, results presented in C are based on an n = 20 and 21, and in D on n = 14 and 11, respectively; values are mean ± SD.