Figure 7.

dPIS is the only PI synthase in Drosophila. A, Depletion of dPIS with anti-dPIS antibodies. A Western blot, consisting of wild-type head and body extracts before and after the dPIS depletion, was probed with anti-dPIS antibodies. The total proteins in each lane were detected by staining with Ponceau S staining (supplemental Fig. 1C, available at www.jneurosci.org as supplemental material). B, PI synthase activities in fly head and body extracts before and after depletion of dPIS. C, CDP–DAG synthase activities in head and body extracts of cds¹ mutant flies compared with wild type. Also shown are the CDP–DAG synthase activities in wild-type extracts before and after depletion of dPIS.