Figure 2.

In mhtt cells, Val-BDNF but not Met-BDNF is retained at the Golgi apparatus. A, B, Colocalization of Val-BDNF or Met-BDNF with calnexin in wt and mhtt cells did not show any difference between all conditions. C, D, Colocalization of Val-BDNF or Met-BDNF with giantin, a Golgi marker, revealed a higher colocalization of Val-BDNF in mhtt cells compared with wt cells, although these effects were not observed in mhtt cells expressing Met-BDNF compared with wt cells. Results are represented as a mean ± SEM determined from analysis of three independent experiments [not significant (n.s.), p > 0.05; ***p < 0.001 vs Val-BDNF in wt cells]. Scale bar, 8 μm.