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Relative expression of nNOS (A) and eNOS (B) during demyelination and remyelination induced by cuprizone in wild-type mice. mRNA was isolated from the corpus callosum of wild-type mice, and quantitative real-time PCR using gene-specific primers for nNOS (A) and eNOS (B) was performed. β-Actin gene was used for normalization. The results are expressed as relative fold change over untreated mice. *p < 0.05; **p < 0.01.
