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. 2006 Nov 29;26(48):12602–12608. doi: 10.1523/JNEUROSCI.4020-06.2006

Figure 4.

Figure 4.

Avidin cross-linking immobilizes EYFP-GIRK1. A, Confocal images of HEK 293 cells expressing EYFP-GIRK1 and unlabeled GIRK4 after cell surface biotinylation (Control) or cell surface biotinylation and avidin cross-linking (Avidin). Cells are shown before and after photobleaching of a 5-μm-diameter ROI (arrow) centered on the plasma membrane. Scale bar, 5 μm. B, Fluorescence intensity in the bleached ROI is plotted versus time during FRAP experiments on control (n = 9) and avidin-cross-linked (n = 9) HEK cells. The black traces represent the mean fluorescence intensity, and the gray traces represent the mean ± SEM.