Figure 6.

Netrin-1 mutant spinal cords show a reduction in cell proliferation but not cell death. A, Ventral spinal cord sections from wild-type animals show proliferating cells labeled by anti-BrdU antibodies in both gray matter (GM) and white matter (WM). B, In netrin-1 mutant spinal cord, the number of proliferating cells is dramatically reduced in both gray matter and white matter. C, Cell death detected by antibodies to activated caspase-3 or TUNEL method while slightly increased, it is not significantly altered in the E17 mutant spinal cord (solid bars) compared with wild type (open bars). Scale bar: A, B, 250 μm. Error bars represent SEM.