Figure 5.

Effect of an anti-actin monoclonal antibody on catecholamine release. PC12 cells were preincubated with either an anti-actin monoclonal antibody (■) or the isotype control (MOPC-21C; □) for 30 min and treated with 60 μm nicotine or untreated at 37°C for 15 min, and catecholamine release was measured as described in Materials and Methods. Results are mean ± SEM; n = 3 for each experimental group. **p < 0.01 for the anti-actin antibody compared with corresponding values for the MOPC-21C isotype control.