Figure 3.

Time-dependent activation of phospho-p70s6K in the amygdala after fear conditioning. Bars represent mean optical density (OD) values (±SEM) expressed as a percentage of control. a, Rats killed at 30 and 60 min after fear conditioning showed significantly more phospho-p70s6K expression in the amygdala compared with home cage (HC) controls or animals killed 10 min after conditioning. This increase in phospho-p70s6K seen 60 min after training is reduced by an immediate posttraining injection of RAP (b). c, d, No differences between groups were observed when the same samples were probed with an antibody for total p70s6K (c), and no changes in total p70s6K expression were seen in the amygdala after RAP infusion (d) (*p < 0.05).