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. 2006 Dec 20;26(51):13328–13337. doi: 10.1523/JNEUROSCI.2858-06.2006

Figure 4.

Figure 4.

OLe contralateral dendrites are missing or fail to cross the midline after knockdown of DCC by DCC–MO2 or DCC–MO3 or knockdown of Netrin1 by coinjection of netrin1a splice-blocking MO (ntn1a-SBMO) and netrin1b–MO (ntn1b-MO). a–f, Confocal projections of the hindbrain of live 36 hpf embryos, reversed contrast. Rostral is up, and caudal is down. Normal positions of contralateral dendrites indicated by arrow (REN) or arrowhead (CEN). a, a′, WT embryos show normal midline crossing by OLe dendrites. b, b′, c, c′, OLe dendrites are strongly reduced by DCC–MO2 (5 ng) and DCC–MO3 (2 ng). d, d′, OLe dendrites are strongly reduced after knocking down both netrin1a and netrin1b by coinjection of netrin1a–SBMO (5.6 ng)/netrin1b–MO (4 ng) or netrin1a–SBMO (9.4 ng)/netrin1b-MO (4 ng). e, Scoring key for a′–d″ (live embryos). Scale bar, 25 μm.