Table 3.

Effect of BDNF on the Q fraction of BrdU⁺ cells

Injection	Area	The ratio of Q fraction of BrdU-positive cells (%)
		12	16	20	24 (h)
Vehicle	VZ	14.1 ± 0.1	31.7 ± 0.3	37.4 ± 1.4	40.1 ± 0.5 (%)
BDNF	VZ	14.9 ± 0.2*	33.1 ± 0.8*	39.4 ± 1.5	40.3 ± 1.0 (%)
Vehicle	Total	21.6 ± 0.5	42.8 ± 1.0	59.1 + 1.7	68.4 + 1.3 (%)
BDNF	Total	24.0 ± 0.5*	47.9 ± 1.5*	63.4 ± 1.1	69.1 ± 0.3 (%)

Single intraperitoneal injection of BrdU was performed 3 h after BDNF or vehicle administration, and then the brains were collected 12, 16, 20, and 24 hr after BrdU injection. The brain sections were double immunostained with antibodies against BrdU and Ki67. The Q fraction in the VZ or in the whole cortex (Total) was determined as ratio (percentage) of the number of Ki67-negative/BrdU⁺ cells to the number of total BrdU⁺ cells. The values are expressed as the mean ± SE.

*p < 0.05, Student's t test; n = 3.