Figure 5.
A, B, c-Fos coordinates target gene expression. We isolated whole-cell extracts from the NAc (A) and CPu (B) of wild-type (+/+) and f/f–Fos–D1–Cre (−/−) mouse after saline (0h; n = 4 mice each), 7 (7d; n = 7 mice each), or 28 consecutive days (28d; n = 5 +/+ and 7 −/− mice) of cocaine injections at the 20 mg/kg dose. We performed Western blotting for the indicated proteins and quantified gene expression levels with saline-treated wild type as 100% controls. Data represent mean ± SEM. *p < 0.05 (for NAc and CPu, F(1,33) = 34.26 and 24.68 for NR1; F(1,33) = 37.71 and 48.19 for GluR2; F(1,33) = 11.06 and 27.52 for β-catenin; F(1,33) = 23.23 and 34.67 for p35; F(1,33) = 12.10 and 38.20 for Cdk5) between genotypes. C, Increasing amounts of Fos (1 and 2) were transfected into various cells in duplicates with mock-transfected cells (M) as controls. Whole-cell extracts were used for Western blotting.