Fig 5. Prior ZIKV infection generates cross-binding and cross-neutralizing DENV antibodies that are boosted after DENV infection.

(A) DENV-specific neutralizing antibody titer of cohort B animals before (open bars) and 28 days after DENV infection (filled bars) determined by a 50% plaque neutralization test. (B) ZIKV-specific neutralizing antibody titer of cohort B animals before and 28 days after infection with DENV determined by a 50% plaque neutralization test. (C) Whole virion DENV-specific binding antibody levels of cohort B animals expressed as log₁₀ 50% effective dilution of binding in an ELISA assay before and 28 days after ZIKV infection. (D) Whole virion ZIKV-specific binding antibody levels of cohort B animals before and 28 days after ZIKV infection.