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. 2019 Aug 1;14(8):e0220056. doi: 10.1371/journal.pone.0220056

Fig 7. IFT88 shRNA shortens cilia and enhances RGC degeneration following ONT.

Fig 7

(A) Left: Micrographs of flatmounted retinas showing ACIII immunolabeling of primary cilia in RBPMS-positive RGCs. Right: Micrographs showing GFP positive RGCs expressing AAV2 vectors encoding IFT88 shRNA. Scale bars = 10 μm. (B and C) The length of RGC cilia (B) and the proportion of ciliated RGCs (C) decreased at 3 weeks after AAV2-IFT88-shRNA vector injection. **p<0.01. (D) Representative epifluorescence micrographs of RBPMS immunolabeled RGCs in the inner eccentricity of flatmounted retinas at 7 days post-ONT, expressing control shRNA (top) or IFT88 shRNA (bottom). Scale bar = 100 μm. (E) Quantification of RGCs showing that IFT88 shRNA expression significantly decrease RGC survival at 7 days post ONT, relative to scrambled control hairpin. **p<0.01, n = 4 retinas.