Fig 6. Microbiome analysis.

IgG^hi sorted fecal bacteria from (n = 10) dogs with IBD, and non-sorted bacteria (n = 10; paired fecal samples from dogs with IBD) and bacteria from healthy control animals (n = 10) were analyzed by 16S rRNA sequencing, as described in Methods. (A) Species abundance heat map at taxonomic level representing average differences, with 0 = no difference, -1 and 1 representing maximum differences. (†) Showing the top 10 taxa abundance. (B) Bar graph depicting the relative abundance of 5 major phyla comparing the IgG^hi sorted population with non-sorted bacteria, obtained from same dogs with IBD. A significantly increased abundance of Actinobacteria phyla was found in IgG^hi sorted population. (C) Bar graph showed relative abundance comparing between IgG^hi sorted and non-sorted bacteria for members of Actinobacteria phyla. The data were reported as Mean ± SD, and statistical comparisons were calculated using paired t-test (*P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001).