Shown for three (of ten total) time points surrounding influenza vaccination: two days before, three days after, and seven days after; for partis (top) and TIgGER (bottom). The phylogenetic tree is constructed with a leaf for each germline gene that was inferred at any of the three time points. Branch lengths connecting different V gene families are set to zero. Leaves are colored according to the number of time points at which the corresponding gene was inferred: one (dark grey), two (light grey), or all three (white). Since this data is not IgM specific, IgDiscover is not shown. See Fig 9 for other results for these subjects. The same trees, but with leaves labeled with gene names, are shown in S7 Fig.