Figure 6.

Expression of ankyrin-G has very small effects on coexpressed KCNQ2/KCNQ3 channels. A, Representative current traces in response to pulse protocols (shown in insets) used for kinetic analysis: i, deactivation protocol; ii, activation protocol. B, Analysis of voltage gating of wild-type KCNQ2/KCNQ3 heteromeric channels, C3 motif mutants, and wild-type channels coexpressed with ankyrin-G-GFP: i, deactivation time constants; ii, conductance/voltage curves; iii, activation time constants. The kinetics of the three conditions is very similar, with some evidence for slight slowing of activation and deactivation gating in the presence of ankyrin-G. Steady-state voltage dependence of gating of wild-type and mutant are indistinguishable, but ankyrin-G confers a ∼6 mV shift toward depolarized voltages.