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. 2006 Mar 8;26(10):2673–2683. doi: 10.1523/JNEUROSCI.4689-05.2006

Figure 2.

Figure 2.

Selective AMPA and NMDA antagonists differentially suppress glutamate-induced current in single astrocytes. A, NBQX inhibits the fast component of glutamate-induced current. Representative traces illustrate the current before, during, and after application of 30 μm NBQX (left) and the NBQX-sensitive current obtained by subtraction (middle). The right panel shows the concentration dependence of the block of the fast component for four cells (IC50, 2.2 ± 0.4 μm; Hill coefficient, 1.9). B, d-AP-5 inhibits the slow component of glutamate-induced current. Representative traces demonstrating the effect of 1 μm d-AP-5 (left) and the d-AP-5-sensitive component obtained by subtraction (middle) are shown. The right panel shows the concentration dependence of the block for five cells (IC50, 0.64 ± 0.1 μm; Hill coefficient, 1.6). Error bars represent SD. C, NBQX (30 μm) and d-AP-5 (10 μm) applied together inhibit both the fast and slow current components. The residual current is sensitive to glutamate transporter blocker dl-TBOA (100 μm). The inset shows the trace of dl-TBOA-sensitive component of residual current obtained by digital subtraction.