Figure 5.

Combined in situ hybridization and immunocytochemistry verifies that D₂ and D₃ immunoreactivities (IRs) are found in neurons that coexpress transcripts encoding these proteins. In situ hybridization is illustrated in the left panels (A, C, E, G), and immunocytochemistry is illustrated in the right panels (B, D, F, H). Transcripts encoding D₂ (A) and D₂ immunoreactivity (B) are found in the same neurons. A sense probe (control; C) does not label D₂-immunoreactive neurons (D). Transcripts encoding D₃ (E) and D₃ immunoreactivity (F) are found in the same neurons. A sense probe (control; G) does not label D₃-immunoreactive neurons (H). MP, Myenteric plexus.