Figure 4.
Western blot and RT-PCR analysis of dhh+/− and dhh−/− sciatic nerves. A, Western blot analysis of MBP, P0, β-catenin, MAG, and E-cadherin in sciatic nerves from dhh+/− and dhh−/− mice at P10, P21, P30, and 3 months. β-Tubulin is included to show that samples are equally loaded. Note that MBP levels in dhh−/− nerves are lower at 3 months than in dhh+/− nerves, whereas other proteins are unchanged. B, Western blot analysis of cx29 at P21. Note the upregulation of a strong band at ∼60 kDa and a less prominent band at ∼29 kDa in the dhh−/− nerves. C, RT-PCR of mRNA in sciatic nerves from dhh+/− and dhh−/− mice at P10, P21, P30, and 3 months of molecules that are expressed either in SLI and/or endothelial junctions. GAPDH is included to show that samples are equally loaded. Note that cx29 mRNA levels are increased in dhh−/− nerve at 3 months.