Figure 6.
Dhh−/−/shi−/− double null sciatic nerves have increased numbers of SLI. A, B, Semithin toluidine blue-stained sections of wild-type (A) and dhh−/−/shi−/− (B) nerves. Note the arrangement of the double null nerve into mini-fascicles, a phenotype typical of dhh−/− null nerves. C, Teased preparations of wild-type, shi, and double null nerves from 3.5-month-old mice, using phalloidin to label SLIs. The double null nerve fibers have many more SLIs than the wild-type ones. D, Electron micrograph showing a complex SLI in a transverse section of double null nerve. E, Electron micrograph of multiple SLIs (arrows) in a double mutant nerve shown in a longitudinal section. Scale bars: A, B, 70 μm; C, 66 μm; D, 0.9 μm; E, 0.7 μm.