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. 2006 May 31;26(22):5996–6003. doi: 10.1523/JNEUROSCI.5380-05.2006

Figure 3.

Figure 3.

Effect of conditioned medium on neurosphere migration. A–E are images of migration of neural progenitor cells out of neurospheres cultured with conditioned medium harvested from MBECs (A), MBECs and rhEPO (B), MBECs and rhEPO in the presence of OA Hy (C), LY294002 (D), or U0126 (E). F and G are quantitative data of average distance and farthest distance of neural progenitor cell migration. ∗p < 0.05 versus the control group and #p < 0.05 versus the rhEPO group. H shows quantitative data of fold changes of neural progenitor cell migration by blind-well chamber assay. ∗p < 0.01 and #p < 0.01 versus control and rhEPO groups, respectively. Error bars indicate SEM. I and J are images of double immunofluorescent staining showing TuJ1 (red) and GFAP (green)-positive cells in neural progenitor cells cocultured with endothelial cells treated with (I) and without rhEPO (J). OA, OA Hy; GM, GM6001; LY, LY294002; U, U0126. Scale bar, 100 μm.