Figure 4.

Effect of pre-reactivation intra-BLA Zif268 ASO on the subsequent maintenance of cocaine seeking under a second-order schedule of reinforcement. The number of active lever presses during the first, pre-cocaine interval (a) and averaged over the four post-cocaine intervals (b) are presented for representative sessions in the four stages of the experiment (n = 6–10 per group): baseline, CS introduction under a second-order schedule of reinforcement [FI15(FR10:S); session 6 after reactivation], CS omission (FI15; session 13), and CS reintroduction (session 15). Groups were infused with Zif268 ASO or MSO and reexposed to either the training context alone (Non-reactivated) or the cocaine-associated CS in the training context (Reactivated). Infusion and reexposure took place between baseline and CS introduction. Data are presented as mean ± SEM.