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alx-positive neurons are likely to be glutamatergic. Images were taken from 32 hpf embryos. A, Dual in situ hybridization of alx and vglut2. All of the alx-positive cells are vglut2 positive. B, Dual in situ hybridization of alx and glyt2. None of alx-positive cells are positive for glyt2. Scale bar, 20 μm.
