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. 2006 May 17;26(20):5370–5382. doi: 10.1523/JNEUROSCI.5255-05.2006

Figure 7.

Figure 7.

GABAB receptor activation is involved in NMDA- and baclofen-induced depression. A, Data from a single slice (top panel) and for all slices (bottom panel; n = 6 slices) showing changes in fEPSP amplitude as percentage of baseline before, during, and after NMDA bath application (25 μm; 3 min) (bar) in paired control experiments (gray) and in the presence of the GABAB receptor antagonist CGP55845 (2 μm, black). Summary bar graph (inset) shows the significant reduction of NMDA-induced depression by CGP55845 (Student’s paired t test; *p < 0.01). Top panel insets show examples of fEPSPs before (1) and during (2) synaptic depression in control, and in the presence of CGP55845 (3). Calibration: 0.5 mV, 20 ms. B, Relative increase (mean ± SEM) in fluorescence for glial cells in a single slice (top panel; n = 7 cells) and for all slices (bottom panel; n = 47 cells, 7 slices) before, during, and after NMDA bath application (25 μm) in control (gray) and in the presence of CGP55845 (2 μm; black). Summary bar graph (inset) shows the blockade of NMDA-induced glial Ca2+ responses by CGP55845 (Student’s paired t test; *p < 0.001; same experiments as in A). C, Data from single slice (top panel) and for all slices (bottom panel; n = 6 slices) showing changes in fEPSP amplitude as percentage of baseline before, during, and after bath application of the GABAB receptor agonist baclofen (20 μm; 3 min) (bar) in paired control experiments (gray) and in the presence of CGP55845 (2 μm; black). Bar graph (inset) depicting the baclofen-induced reduction in fEPSP amplitude (gray) blocked by the GABAB receptor antagonist CGP55845 (black; Student’s paired t test; *p < 0.01). Top panel insets show examples of fEPSPs before (1) and during (2) synaptic depression in control, and in the presence of CGP55845 (3). Calibration: 0.2 mV, 20 ms. D, Relative increase (mean ± SEM) in fluorescence for glial cells in a single slice (top panel; n = 5 cells) and for all slices (bottom panel; n = 33 cells, 6 slices) before, during, and after baclofen bath application (20 μm) in control (gray) and in the presence of 2 μm CGP55845 (black; paired experiments; same experiments as in C). Bar graph (inset) shows the average baclofen-induced Ca2+ responses in control and in the presence of CGP55845. Glial Ca2+ responses were prevented by the GABAB receptor antagonist (Student’s paired t test; *p < 0.01). Error bars indicate SEM. BAC, Baclofen; Ctrl, control; resp., response.