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Discharge profile of a GAD+ cluster-firing neuron recorded in an unanesthetized rat (A). Note that the pattern of discharge is maintained when the vigilance shifts from W to SWS. B, Single confocal sections showing the neuron A labeled with neurobiotin (Nb; red). This neuron was also immunoreactive for GAD (blue). Scale bar, 20 μm. The red dot on the frontal section of MS-DB shows the location of the neuron.
