Figure 4.

Extinction training plus DCS reverses the conditioning-induced increase in the surface expression of GluR1. A, Representative blots and mean ± SEM of GluR1 immunoreactivity. Rats were conditioned, and 24 h later they were infused with DCS (10 μg per side) bilaterally into the amygdala 30 min before or after extinction training. Twenty-four hours after extinction training, LA and BLA tissues were dissected out and the surface GluR1 levels were determined using biotin labeling. Cotreatment of DCS with light-alone trials blocked the conditioning-induced increases in surface expression of GluR1. Intraperitoneal HA-966 (6 mg/kg) 30 min before DCS blocked the effect of DCS. *p < 0.05 versus extinction. B, Extinction training plus DCS treatment did not influence surface GluR1 levels in control rats that were given light and footshock in an unpaired manner (unpaired) or received neither light nor footshock (naive). Ext, Extinction; Veh, vehicle; Unp, unpaired; HA, HA-966.