Figure 5.

DCS facilitates LFS-induced depotentiation and reduction in surface levels of GluR1. A, The graph represents the mean ± SEM slope of fEPSPs plotted against time. LFS applied 60 min after the TS had no lasting effect on the LTP. However, in the presence of DCS (10 μm), LFS induced depotentiation. B, Amygdala slices were randomly assigned to the naive, TS, LFS 60 min, and LFS 60 min plus DCS groups. Slices were given five sets of TS at an interstimulus interval of 1 min to the EC in the TS group. In the LFS 60 min group, LFS was delivered at 60 Hz after the onset of TS. DCS was applied 5 min before and during the LFS in the DCS plus LFS 60 min group. Two hours after the onset of TS, a relatively small portion of the LA was dissected from each slice. Four to six slices from each animal were pooled together for biochemical analysis. Naive control slices were incubated in ACSF for 2 h. ***p < 0.001 versus TS.