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. 2006 Jan 18;26(3):1006–1015. doi: 10.1523/JNEUROSCI.2806-05.2006

Figure 6.

Figure 6.

SSH1 and LIMK are regulated by Nogo-66 in a ROCK-dependent manner. A, Phospho-LIMK levels in PC12 cells stimulated with Nogo-66. B, V5-SSH and actin content in immune complexes isolated with V5 agarose after Nogo-66 stimulation of PC12 cells. C, Quantification of phospho-LIMK levels in response to AP-Nogo-66 stimulation. Values were normalized to total actin in the lysates. D, Quantification of actin content in V5-SSH1 immune complexes in response to AP-Nogo-66. Actin levels were normalized to the V5SSH1 content for each immunoprecipitate. E, Phospho-cofilin levels in PC12 cells treated with AP-Nogo-66. For A, B, and E, dependence on ROCK was assessed with a 30 min pretreatment of the cells with 10 μm Y-27632. F, Quantification of phospho-cofilin levels in Y-27632-treated PC12 cells and in PC12 cells treated with AP-Nogo-66 after a 30 min pretreatment with 10 μm Y-27632. IP, Immunoprecipitation. Error bars represent SEM.