Figure 10.

Activation of Erk by forskolin in rat and mouse spinal cord slices. Forskolin-induced pErk immunoreactivity in dorsal horn neurons in spinal cord slices from adult rats (C) and mice (D) compared with the control (A and B, respectively) is shown. Pooled data from rats and mice are shown in E. F, Pretreatment with an intrathecal injection of forskolin (12 nmol) 30 min before the unilateral hindpaw injection of CFA in AC1&8 DKO mice rescued mechanical allodynia (n = 4 mice in each group). Behavioral responses to the von Frey stimulation (filament No 2.44) on the dorsum of the ipsilateral hindpaw are plotted against time. Mechanical allodynia was tested on days 1, 3, and 5. Asterisks indicate significant differences compared with control; *p < 0.05; **p < 0.001. Scale bars, 100 μm. Error bars indicate SEM.