Figure 7.

Effects of activation of mGluR5 with DHPG on phosphorylation and expression of key transcription factors downstream to the JNK pathway in cultured rat striatal neurons. DHPG increased cellular levels of phospho-c-Jun at serine 63 (A), c-Jun (B), and JunD (C) in a time-dependent manner. Total levels of ATF-2 (D) and β-actin (E) proteins remained unchanged throughout the time course surveyed. Western blots were probed with the indicated antibodies on the lysates from striatal cultures treated with DHPG at 100μm for different durations. Representative immunoblots are shown to the left of the quantified data (mean ± SEM; n = 3–6). *p < 0.05 versus corresponding basal levels.