Skip to main content
. 2019 Aug 1;14(8):e0220475. doi: 10.1371/journal.pone.0220475

Fig 5. Determination of the optimal number of reference genes for normalization.

Fig 5

(A) Developmental stages of G88 strain; (B) Tissues of G88 strain; (C) Tissues of GC strain. Pairwise variations (Vn/n+1) were calculated between the normalization factors NFn and NFn+1 by geNorm to determine the optimal number of reference genes. geNorm decides whether inclusion of an extra reference gene adds to the stability of the normalization factor.