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. 2006 Feb 1;26(5):1516–1530. doi: 10.1523/JNEUROSCI.4543-05.2006

Figure 5.

Figure 5.

Long-term overexpression of GFP–TrkB.T1 causes neuronal atrophy and dendritic beading in facial motor neurons. Transgene expression was detected with GFP immunohistochemistry. A, Transduced cells at 2 weeks of expression of GFP–TrkB.T1 show typical motor neuron morphology (arrows). B, At 4 weeks, some GFP–TrkB.T1-expressing neurons exhibit a reduced soma size (arrowheads), whereas other neurons are not affected (arrows). C–E, Transgene expression at 2 months. C, Example of a motor neuron with a rounded soma (open arrowhead) and severe dendritic swelling (indicated with arrowheads). Neighboring transduced neurons display regular soma contours and dendrites (arrows). D, GFP–TrkB.T1-expressing motor neuron with a rounded soma (open arrowhead). Dendrites contain large, spherical swellings (arrowheads) separated by thin, atrophied segments. E, Higher magnification of the boxed area in D. Open arrowheads indicate dendritic varicosities, which appear as elongated enlargements of dendrites connected by segments of normal caliber and also occur in intact motor neurons. F, GFP–TrkB.T1 motor neuron morphology at 4 months (arrows). The open arrowhead indicates transduced motor neuron with rounded soma, eccentric nucleus, and thinned dendrites (arrowheads). G, GFP-f-expressing motor neurons (arrows) show regular soma contours and dendrites (arrowheads) 2 months after injection. Scale bars: (in G) A–C, F, G, 100 μm; D, 25 μm; E, 10 μm.