Early-born (E12.5) interneurons in Dab1 mutant brains show layer
inversion. A, Double-labeling for GABA (green) and
BrdU (red) reveals that the vast majority of double-labeled cells (yellow) in
wild-type cortex are in the lower layers at P16. B,
Higher magnification of boxed area in A indicating
that the majority of double-labeled interneurons (arrows) are in the lower layers of
the cortex. C, Histogram depicting the layer positions
of GABA–BrdU interneurons (n = 3 animals, 147 cells; mean
± SEM percentage) indicating that majority of early-born interneurons in
wild-type cortices are distributed in the lower layers.
D, In Dab1 mutants, double-labeled
interneurons (yellow) born at E12.5 are found in the upper layers.
E, Higher magnification of boxed area in
D indicating that the majority of double-labeled
interneurons (arrows) are in the upper layers of the cortex.
F, Histogram depicting the layer positions of
GABA–BrdU interneurons (n = 3 animals, 246 cells; mean
± SEM percentage) indicating that the majority of early-born interneurons in
Dab1 mutant cortices are distributed in the upper layers. Scale
bar: A, D, 160 μm;
B, E, 80 μm.
WM, White matter.