Table 1.

Effects of temperature at the calyx of Held synapse

Parameter	Average across cells		Paired
	RT	PT	Percentage change	p (paired t test)
P8–P10
EPSC (N = 9)
Amplitude (nA)	−8.0 ± 1.26	−13.8 ± 1.9	75 ± 15	<0.001
10–90% rise time (ms)	0.33 ± 0.025	0.22 ± 0.016	−39 ± 2	<0.001
Half-width (ms)	1.2 ± 0.12	0.71 ± 0.073	−49 ± 3	<0.001
Charge (pC)	−16 ± 2.8	−16 ± 2.5	0 ± 10	>0.05
Quantal content	279 ± 50	284 ± 44	4.0 ± 9.2	>0.05
mEPSC (N = 9)
Frequency (s−1)	4.7 ± 1.3	9.3 ± 1.8	167 ± 38	<0.05
Quantal size (pA)	−53.1 ± 7.5	83.3 ± 12.3	62 ± 10	<0.001
10–90% rise time (ms)	0.30 ± 0.04	0.21 ± 0.02	−26 ± 4	<0.001
Half-width (ms)	0.69 ± 0.09	0.46 ± 0.06	−32 ± 3	<0.001
Charge (fC)	−57 ± 9.3	−57 ± 8.7	5 ± 7	>0.05
Depression (100 Hz; N = 9)
Paired-pulse ratio	0.53 ± 0.10	0.52 ± 0.07	7 ± 11	>0.05
Steady-state EPSC (nA)	−0.31 ± 0.08	−1.8 ± 0.49	486 ± 82	<0.001
Steady-state depression (%)	95 ± 3.3	84 ± 3.8	−12 ± 4	<0.001
qSteady-state (pC)	1.1 ± 0.28	3.0 ± 0.78	198 ± 25	<0.001
Recovery from depression (N = 10)
Percentage fast	16.7 ± 2.0	34.1 ± 4.5	127 ± 30	<0.05
Rate (s−1)	0.61 ± 0.09	1.2 ± 0.14	104 ± 21	<0.01
P16–P18
EPSC (N = 5)
Amplitude (nA)	−8.6 ± 1.9	−14.5 ± 3.0	82 ± 35	<0.05
Charge (pC)	−7.1 ± 2.0	−6.2 ± 1.5	−6 ± 12	>0.05
mEPSC (N = 3)
Quantal size (pA)	−62 ± 16	−88 ± 15	51 ± 18	<0.05
Charge (fC)	−34 ± 8.6	−29 ± 4.7	−10 ± 9	>0.05
Depression (100 Hz; N = 5)
Paired-pulse ratio	0.83 ± 0.08	0.77 ± 0.03	−5 ± 7	>0.05
Steady-state EPSC (nA)	−1.4 ± 0.43	−4.3 ± 1.1	219 ± 45	<0.01
Steady-state depression (%)	83 ± 2.8	70 ± 2.5	−15 ± 2	<0.01
qSteady-state (pC)	2.4 ± 0.61	3.7 ± 0.94	58 ± 21	<0.05
Refilling rate (model fit; s−1)	4.3 ± 0.29	10.9 ± 0.8	157 ± 20	<0.01

For each parameter, the mean ± SEM value taken across cells is given for each temperature condition (RT and PT). On the right, we calculated the average percentage change of each parameter after raising temperature from RT to PT based on a paired comparison within each cell.