Figure 2.

Tat-RACK1 and BDNF induce D₃R expression in the striatum via activation of the TrkB receptor. a, Striatal slices dissected from five rats were preincubated without (lanes 1 and 2) or with 200 nm K252a (lane 3) for 30 min before addition of 50 ng/ml BDNF (lanes 2 and 3) for 1 h. D₃R expression was analyzed by RT-PCR with GPDH control. The histogram depicts the mean ratios of D₃R to GPDH ± SD. n = 5. **p < 0.01 compared with control, or **p < 0.01 comparing BDNF plus K252a with BDNF alone. b, Striatal slices dissected from four rats were treated with 1 μm Tat–RACK1 for the indicated times. D₃R expression was analyzed by RT-PCR with GPDH control. The histogram depicts the mean ratios of D₃R to GPDH ± SD. n = 3. c, Striatal slices dissected from 10 rats were preincubated without or with 200 nm K252a (lanes 3 and 4) for 30 min before addition of 1 μm Tat–RACK1 (lanes 2 and 4) for 4 h. D₃R expression was analyzed by RT-PCR with GPDH control. The histogram depicts the mean ratios of D₃R to GPDH ± SD. n = 6. **p < 0.01 compared with control, or ^##p < 0.01 comparing Tat-RACK1 plus K252a with Tat-RACK1 alone.