Verification of ligand pellet placement. The locations of pellets were
localized in cresyl-violet-stained sections by identifying pellet location
relative to the desired region. A, Typical
histology with hormone-containing pellets indicated.
B–D, Placement of the pellets from a
representative experiment with locations plotted onto atlas diagrams of the
brain in the region of the PVN [diagrams adapted from Swanson (1998/1999)]. Each
black dot represents the center of a hormone pellet. V3, Third ventricle; PVH,
paraventricular hypothalamic nucleus; PVHdp, paraventricular hypothalamic
nucleus, dorsal parvicellular part; PVHmpd, paraventricular hypothalamic
nucleus, medial parvicellular part; PVHpml, paraventricular hypothalamic
nucleus, posterior magnocellular part, lateral zone; PVHpmm, paraventricular
hypothalamic nucleus, posterior magnocellular part, medial zone; PVHpv,
paraventricular hypothalamic nucleus, periventricular part; PVHlp,
paraventricular hypothalamic nucleus, lateral pariventricular part; RE, nucleus
reunions; REd, nucleus reunions, rostral division, dorsal part; REl, nucleus
reunions, rostral division, lateral part; REm, nucleus reunions, rostral
division, median part; PVa, periventricular hypothalamic nucleus anterior part;
PVi, periventricular hypothalamic nucleus, intermediate part; SBPV,
subparaventricular zone hypothalamus; ZI, zona incerta; fx, columns of the
fornix; AHNa, anterior hypothalymic nucleus, anterior part; AHNc, anterior
hypothalymic nucleus, central part; AHNd, anterior hypothalymic nucleus, dorsal
part; AHNp, anterior hypothalymic nucleus, posterior part; RCH, retrochiasmatic
area; sup, supraoptic commissures; ARH, arcuate hypothalamic nucleus; VMHa,
ventromedial hypothalamic nucleus, anterior part; VMHc, ventromedial
hypothalamic nucleus, central part; VMHdm, ventromedial hypothalamic nucleus,
dorsomedial part; VMHvl, ventromedial hypothalamic nucleus, ventrolateral part;
MEin, median eminence, internal lamina; MEex, median eminence, external lamina;
TU, tuberal nucleus; VM, ventromedial nucleus; SMT, submedial nucleus thalamus;
PR, perireuniens nucleus; mtt, mammillothalamic tract.