Table 2.

Primer sequences and annealing temperatures used for amplification of mRNAs for various steroid metabolizing enzymes

Gene	Primers	Annealing temperature	Extension time
17β-HSD	Forward primer, 5′-GTGCGAGAGTCTGGCGATCCTG-3′	68°C	12 s
	Reverse primer, 5′-GGGTAGGAAGCGGTTCGTGGAG-3′
Aromatase	Forward primer, 5′-CCTGGCAAGCACTCCTTATCAA-3′	64°C	22 s
	Reverse primer, 5′-CCTGTGCATTCTTCCGATGTTC-3′
5α-Reductase 1	Forward primer, 5′-CGCGTCCTGCTGGCTATGTT-3′	66°C	19 s
	Reverse primer, 5′-CTGATGGTGCTGCTTCGCTCTG-3′
CYP7B	Forward primer, 5′-AGCTATGGAAGTCCTGCGTGA-3′	62°C	22 s
	Reverse primer, 5′-GCCCAGAAACATGCGACTGT-3′
3α-HSD	Forward primer, 5′-GCAAGATTGAAGACGGCACTG-3′	60°C	1:15
	Reverse primer, 5′-AGCTGGTAGCGAAGGGCAACTA-3′
3β-HSD	Forward primer, 5′-GGCCAGAGGATCATCCGGATGTT-3′	67°C	10 s
	Reverse primer, 5′-TGTCCCGATCCACTCCGAGGTTT-3′